Agarose Gel Electrophoresis

 


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Agarose gel electrophoresis : Overview

Agarose : Structure

Agarose : Properties

Agarose : Applications

Agarose gel electrophoresis : Electroendosmosis (EEO)

Agarose gel

Dissolving agarose in a suitable buffer

Agarose Gel formation (Gelation) - Mechanism

Boiling method for dissolving agarose

Microwave method for dissolving agarose

Agarose concentration vs resolution of linear DNA fragment

Loading a sample in agarose gel

Preparation of agarose gel for DNA analysis

Table : Agarose amount needed to prepare agarose solution of specific concentration and volume

Table : Recommended concentration of agarose gel for separating linear DNA fragments of various sizes

Ethylene diamine tetraacetic acid (EDTA)

Preparation of 500 ml of 0.5M EDTA solution

Preparation of 500 ml of 10N NaOH solution

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